Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 2. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of IM implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: Acta biomaterialia

Article Title: In vivo short-term and long-term host reaction to starch-based scaffolds.

doi: 10.1016/j.actbio.2010.06.020

Figure Lengend Snippet: Fig. 5. Micrographs of the sections of the explanted FB and WS produced starch-based scaffolds after 1 (A–D) and 2 (E–H) weeks of SC implantation in rats. Tissue was immunohistochemically labelled for CD18 and CD3. represents the SPCL scaffold fibres, arrows mark CD18-positive cells and dashed arrows mark CD3-positive cells.

Article Snippet: The histological analysis of crosssection samples was performed after haematoxylin and eosin (HE), and Masson Goldner Trichrome (MGT) staining and immunohistochemistry using a monoclonal mouse anti-human CD3 antibody (Dako, Denmark) with cross-reactivity for rat T lymphocytes, a monoclonal mouse anti-rat CD18 antibody (Serotec, UK) for the integrin b2 chain of recruited leukocytes, and a monoclonal mouse anti-human phosphoinositide 3-Kinase (Pi3K) antibody (BD, Belgium) with cross-reactivity for rat activated and proliferating lymphocytes, following standard protocols.

Techniques: Produced, Starch

Journal: BMC Physiology

Article Title: The tripeptide feG ameliorates systemic inflammatory responses to rat intestinal anaphylaxis

doi:

Figure Lengend Snippet: Activation markers on interstitial cells. Effects of feG on staining of interstitial leukocytes in the jejunal muscle for CD18 and ED9 antigens.

Article Snippet: The intestinal tissues were incubated for 24 h at 4°C with either ED9 or CD18 primary antibody (Cedarlane, Hornby, ON) at a 1:100 dilution) followed by three 5-min washes in PBS.

Techniques: Activation Assay, Staining, Control

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Liver-Specific siRNA-Mediated Stat3 or C3 Knockdown Improves the Outcome of Experimental Autoimmune Myocarditis

doi: 10.1016/j.omtm.2020.05.023

Figure Lengend Snippet: Reduced Heart Infiltration and Damage in Healthy C5 −/− and C3 −/− /C5 −/− S tat 3 C/C Mice Heart infiltration was analyzed by flow cytometry and immunohistochemistry in mice of the different genotypes, described in Figure 1 , either healthy (striped bars) or diseased (filled bars). (A) Heart-infiltrating CD11b + cells, either positive or negative for Gr1, were analyzed by flow cytometry. Data are mean ± SEM of the indicated values. Mann-Whitney U test, ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, not significant. (B) Hearts were dissected, formalin fixed, sectioned, and analyzed by hematoxylin and eosin (H&E), CD18 antibody (pan-monocyte marker), or picrosirius red staining (collagen 1 and 3). Scale bars, 100 μm. Representative samples are shown. (C) The infiltrated area was measured on H&E sections in at least 3 mice/group and expressed as percentage of heart area, as described in . Data are mean ± SEM of the indicated values. n.d., not detected. Unpaired two-tailed Student’s t test with Welch’s correction, ∗p < 0.05; ∗∗p < 0.01, ∗∗∗∗p < 0.0001.

Article Snippet: For immunohistochemistry, antigen retrieval on formalin-fixed paraffin-embedded (FFPE) sections was performed with citrate buffer (Sigma-Aldrich), and sections were then saturated (3% BSA + 5% goat serum in Tris-buffered saline [TBS]); incubated overnight with rat anti-mouse CD18 (cat. T-2122; BMA Biomedicals, Augst, Switzerland), followed by biotinylated anti-rat IgG (cat. BA-2000; Vector Laboratories) and avidin-biotin (ABC)-HRP complex (cat. PK-4000; Vector Laboratories); and developed with 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate (sub: cat. 11718096001; Roche Diagnostics, Mannheim, Germany).

Techniques: Flow Cytometry, Immunohistochemistry, MANN-WHITNEY, Marker, Staining, Two Tailed Test

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Liver-Specific siRNA-Mediated Stat3 or C3 Knockdown Improves the Outcome of Experimental Autoimmune Myocarditis

doi: 10.1016/j.omtm.2020.05.023

Figure Lengend Snippet: siRNA-Mediated Liver-Specific Downregulation of C3 or Stat3 Can Prevent Myocarditis in S tat 3 C/C Mice Newborn S tat 3 C/C mice were injected i.p. (from postnatal day 0 to 21) and then i.v. every 3 days with siRNA control (CTRL), against C3 or against Stat3, followed by serum withdrawal and heart analysis, as described below. (A) The Kaplan Meyer curve shows survival of S tat 3 C/C mice injected with siRNA CTRL (gray line, n = 26), against C3 (orange line, n = 9) or against Stat3 (purple line, n = 7). Log rank Mantel-Cox test, p values: CTRL siRNA versus C3 siRNA, ∗∗∗p < 0.001; CTRL siRNA versus Stat3 siRNA, ∗∗p < 0.01. (B) Serum anti-myosin IgG1 autoantibodies were measured by ELISA in mice treated with CTRL siRNA (gray bar); C3 siRNA, either diseased (filled orange bar) or healthy (striped orange bar); and Stat3 siRNA, either diseased (filled purple bar) or healthy (striped purple bar). Data are mean ± SEM. Unpaired two-tailed Student’s t test with Welch’s correction,: ∗∗p < 0.01; ∗∗∗p < 0.001. (C) The hearts of mice belonging to the above groups were sectioned and analyzed by H&E, CD18 antibody (pan-monocyte marker), or picrosirius red staining (collagen 1 and 3). Scale bars, 100 μm.

Article Snippet: For immunohistochemistry, antigen retrieval on formalin-fixed paraffin-embedded (FFPE) sections was performed with citrate buffer (Sigma-Aldrich), and sections were then saturated (3% BSA + 5% goat serum in Tris-buffered saline [TBS]); incubated overnight with rat anti-mouse CD18 (cat. T-2122; BMA Biomedicals, Augst, Switzerland), followed by biotinylated anti-rat IgG (cat. BA-2000; Vector Laboratories) and avidin-biotin (ABC)-HRP complex (cat. PK-4000; Vector Laboratories); and developed with 3,3′-diaminobenzidine tetrahydrochloride (DAB) substrate (sub: cat. 11718096001; Roche Diagnostics, Mannheim, Germany).

Techniques: Injection, Control, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Marker, Staining

Journal:

Article Title: Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina

doi:

Figure Lengend Snippet: CD18 blockade prevents leukocyte adhesion and endothelial cell injury and death in diabetic retinal venules and capillaries. A: After treatment with anti-CD-18 F(ab′)2, diabetic retinal leukocyte adhesion was markedly decreased in the retinal venules and capillaries. B: Analysis with PI revealed a concomitant decrease in retinal endothelial cell injury and death; however, no change in arteriolar endothelial cell injury and death was observed.

Article Snippet: In separate experiments, diabetic rats received either 1 mg/kg of a mouse anti-rat CD18 F(ab′) 2 (WT.3) (Associates of Cape Cod Inc., Falmouth, MA) or a purified mouse anti-human non-immune F(ab′) 2 (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

Techniques:

Journal:

Article Title: Leukocyte-Mediated Endothelial Cell Injury and Death in the Diabetic Retina

doi:

Figure Lengend Snippet: Anti-CD18 surface staining of a static leukocyte within a vessel. Green fluorescence from the FITC-linked Concanavalin A (A) and red fluorescence from the anti-CD18 antibody (B) identify the leukocytes as being CD18-positive when the images are superimposed (C).

Article Snippet: In separate experiments, diabetic rats received either 1 mg/kg of a mouse anti-rat CD18 F(ab′) 2 (WT.3) (Associates of Cape Cod Inc., Falmouth, MA) or a purified mouse anti-human non-immune F(ab′) 2 (Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

Techniques: Staining, Fluorescence